Volume 9, Issue 1, Autumn 2018, Page 1-92


Prevalence of seropositive toxoplasma cases in association with the frequency of abortion in sheep and goat

Abdullah O. AL-Hatami; Ihsan K. AL-Kardhi; May A. AL-Mosa

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 1-10

Toxoplasmosis are a serious public-health problem inhuman and animals especially in developing countries, it appear approximately thirty percent from all cases and have higher level of morbidity .At every stage of life the Toxoplasma gondii infections are a significant cause .The epidemiology and handling of this condition are various in the developing world, where infectious agents became predominate .This study was proceed during the period from March 2017 to August 2017 to detect Toxoplasma gondii in sheep and goat serum by characterize them using two assays Latex agglutination test and IgG and IgM Toxoplasmosis ELISA kits.
A total of 74 sera samples were collected from sheep and goat from different ages ranged 6 month to 2.5 years old were achieved in different reign in AL- Najaf city / Iraq ,they suffered from endemic abortion and still birth. The overall prevalence of Toxoplasma gondii in were32(62%),18(75%) by Latex agglutination test for sheep and goat, respectively, with non-significant variation (P>0.05) between the two them ,The serum are arranged as follows in a total of 23 (71.8%) and 9 (28.2%)abortion and still birth of sheep ,respectively, and17(94.4%) 1(5.6%) aborted and still birth of goat ,respectively, with significant differences (P≤0.05) between the two them. Out of 32 serum positive of Toxoplasmosis in sheep by using Latex agglutination test, there were 23(71,9%) and 11(43,4%) positive serum by using IgG and IgM ELISA kits, respectively, On the other hand out of 18 toxoplasmosis parasite in goat have positive result in Latex agglutination test, there were 11(61,1%) and 6(33,3%) by using IgG and IgM ELISA kits, respectively, our study was accelerated due to endemic clinical abortion and instill birth for sheep and goat and narrowing functional range of molecular assays by time consumption, in this regard the aim of our study used two functional assay diagnosis Latex agglutination assay and ELISA techniques with increase the accuracy for the detection of the Toxoplasmosis in sheep and goat.

Hemato-Biochemical and Histopathological Changes Caused by Coccidiosis in broilers.

Haider A. H. AL-Saegh

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 11-20

The aim of present work is to determine the Hematological, biochemical and histopathological alterations caused by coccidiosis in broiler chickens from an outbreak of bloody coccidiosis in a flock. The study was conducted on Al bahrani field for commercial broiler in Al Najaf province-Iraq.
Blood samples were collected from jugular vein into EDTA tubes for hematological value and plain tube for biochemical value during October 2017 . Result showed that the Anemia caused by the coccidian was characterized by a decreased number of red blood cells (RBC) and decreased packed cell volume (PCV). Differential leukocyte counts revealed to increase in monocytes, lymphocytes, heterophil and eosinophil. Also, Serum biochemical analysis showed decreases in alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and a marked increase in alkaline phosphatase (ALP) activities. On the other hand, Histopathological examinations of the affected caeca also demonstrated excessive tissue damage, hemorrhage, the crypt cells were highly invaded with the developmental stages of E. tenella schizonts and gametocytes that their morphology is practically missing. In conclusions the present study revealed changes in the hematology, blood chemistry and histopathology of broilers caused by E. tenella and E.brunetti.

Dermatitis associated with the Ornithonyssus bacoti

Sundus N. Al-Huchaimi

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 21-25

Cutaneous lesions caused by Ornithonyssus bacoti are misdiagnosed as other infection with bacteria or fungi or as allergies . Dermatitis in the persons working in animal house was infested with O. bacoti is reported in this study .A small number of studies confirmed that the Ornithonyssus species may cause dermatitis in human. The physical examination for patients showed separate papules scattered over their ( arms, shoulder ,legs) , restlessness and urticaria -like dermatitis Microscopic examination was performed for 80 parasite from rat in animal house and 20 from clothing of researchers that worked in animal house and confirmed by PCR amplification.

Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria

Saadia Saleh Mehdy Al-zeiny

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 26-34

Peganum harmala L. (Zygophyllaceae) used in traditional medicine of Iraq and itis one of the most famous medicinal plants. The most bacteria pathogen is resistance for synthesis antibiotic drugs resulting from abuse uses of the patient or produce resistance strains for microorganism therefor, the aim of this search is the effect antibacterial of seed alcoholic extract of peganum harmala on some Gram negative bacteria pathogen. Methods: The broth serial dilution assay method determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of seed methanolic extract of Peganum harmala were tested for antibacterial effect against bacterial isolates (salmonlla enteric , klebsiela pneumonia multidrugresitant E. Coli and highly resist E.coli), used phytochemical analysis of seed mathanolic extrac of peganumharmala. Result: showed good antibacterial activity against strain Salmonella enteric and Klebsella pneumonia, were MBC at concentration (25 mg/ml) and no effect of low concentration of MIC. The MBC value against strain multidrugresistant Escherichia coli, was at concentration (3.125 mg/ml) and MIC at (25 and 12.5 mg/ml), while MBC of highly resist Escherichia coli was in (12.5mg/ml) and MIC value at 50 mg/ml compared with standard antibiotics synthesis. Phytochemical analysis of component seed methanolic extract of peganumharmala showed presence of flavonoids, alkaloids, saponins, tannins, glycosides, terpenoids and steroids.

Protective role of soybean lecithin in reducing hypercholesterolemia and DNA fragmentation inducing by high cholesterol in adult male rats

Sarah Mohammed Alshammary; Luma Waleed Khaleel

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 35-45

The present experiment was investigated to study the ameliorative role of soybean lecithin specially phosphatidylcholin on some lipid profiles, glycemic index and DNA damage in intact and hypercholesterolemic infected rats.Thirty two adult male rats have been used in this study, were randomly selected and equally divided in to four groups as follows C, T1 , T2, T3.They were treated orally (daily) for 42 days as follows; C:control group, were given distilled water by gavage needle, rats of this group were given soybean lecithin only (430mgkgday) orally; T2:rats of this groups were given only cholesterol (10gmday) orally; T3: rats of this groups were given soybean lecithin (430mgkgday) orally, and high cholesterol (10gmday) orally.The daily supplementation of soybean lecithin induces a significant decrease (p > 0.05) in total cholesterol (TC) and triglyceride (TG) in both intact and hypercholesterolemic infected rats respectively.Moreover,the glycemic index reveals a significant decrease in both glucose concentration and insulin resistance, whereas elevation in the concentration of insulin hormone significantly was noticed.In T3 group the results of Agarose Gel Electrophoresis showed that animals given Cholesterol plus soybean lecithin showing improvement in DNA damage by decreasing the fragmentation and increasing the condensation and concentration.In conclusion, the results from this experiment confirm that soybean lecithin supplementation to rats has an important protective role on cardiovascular system and liver in hypercholesterolemic infected rats.This supplementation can overcome the deleterious effect of hypercholesterolemia on heart and liver basically.

Ameliorative effect of soybean lecithin on the liver enzymes of rats supplemented with high cholesterol

Sarah Mohammed Alshammary; Luma Waleed Khaleel

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 46-53

The present experiment was investigated to study the ameliorative role of soybean lecithin specially phosphatidylcholin on liver enzymes (ALT) and (AST) in intact and hypercholesterolemic infected rats.Thirty two adult male rats have been used in this study, were randomly selected and equally divided in to four groups as follows C, T1 , T2, T3.They were treated orally (daily) for 42 days as follows; C:control group, were given distilled water by gavage needle, T1: rats of this group were given soybean lecithin only (430mgkgday) orally; T2:rats of this groups were given only cholesterol (10gmday) orally; T3: rats of this groups were given soybean lecithin (430mgkgday) orally, and supplemented with high cholesterol (10gmday) orally within diet.The daily supplementation of soybean lecithin induces a significant decrease (p > 0.05) in liver enzymes (ALT & AST) as compared with (T2) group which has seen remarkable rise in these enzymes respectively. Moreover, the histopathological examination showed results referred that cholesterol effects significantly on the liver cells by causing sever fatty changes characterized by variable size round clear spaces in the hepatocytes. Also, there is a mononuclear cells aggregation in liver parenchyma and congested blood vessels without any fatty vacuoles in (T2)..In conclusion, the results from this experiment confirm that soybean lecithin supplementation to rats has an important protective role on hepatic portal system in hypercholesterolemic infected rats.This supplementation can overcome the deleterious effect of hypercholesterolemia on liver basically.

Effect of feeding camel colostrum extract on some Reproductive Hormones in Female Rats

Shaimaa Abbas Sabeeh

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 54-58

Finding natural products to prevent or reduce the reproductive system problems is still a goal to many researchers. Natural substances are characterized by their high safety margins
compared to the synthetic ones. The aim of this research was to focus on the effect of camel colostrum extract feeding on some reproductive hormones represented here by Follicle-stimulating hormone ( FSH ) & Luteinizing hormone ( LH ) in female rats. Amounts of 30 ml each of Iraqi camel colostrum were collected post-parturition at 0, 6, and 12 hours in sterile containers and sent directly in icebox to a laboratory. The colostrum samples were centrifuged twice at 5000 rpm for 10 minutes. The supernatants were difiltrated, and the extracts were refrigerated at 4ºC until use. Twenty albino rats were grouped randomly into four equal groups (five rats per group). The control group (CG) was given tap water only, while the treatment groups (0 G, 6G, and 12G) were given 0.1 ml of the extract orally for one week. After 30 days, the rats were sacrificed. Then quickly, blood samples were collected and immediately spun using a centrifuge at 5000 rpm for 5 minutes to collect serum. Finally, serum was measured for the level of FSH and LH. The levels of the FSH & LH showed significant increases in all treated groups compared to the control group

Dexaject dosage for carriage horses: side effects & fixing

Abdul-Samad Uleiwi Hassan; Ali Hussein Aldujaily; Shatha Atta Abeed

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 59-67

The instant study clarify excellent treatment response of dexaject with a dose 3.5/2 mg-ml
in fixing musculoskeletal injuries imaged by x-rays to get a statistical probability less than 0.001
(P<0.001) put it in progress for uses without serious side effects mentioned or noticed in carriage
horse models

Morpho-Histological Investigation of the Kidney of Iraqi Black-Tailed bull Gazelle (Gazella Subgutturosa)

Mohammed; S. Kadhim; Ali; F. Reshag

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 68-75

Morphological and histological characteristics of the kidneys in the Iraqi black-tailed gazelle were investigated by using the morphological and histological methods. The two kidneys in the gazelle contributed about 0.6% of the body weight. The ratio of thickness of the renal medulla to the cortex was 4:1, which indicated that Henle’s loops in gazelle’s kidneys were very long. In cortex, there were more mid-cortical than the juxtamedullary nephrons which had longer Henle’s loops. In the outer medulla, the vase recta were grouped obviously into specific vascular bundles which distributed within the bundles of Henle’s loops and collecting tubules. The inner medulla was thicker than the outer one. Specialized cup like structures were formed by projecting of the pelvis and extended to fit the apex of the (8-10) renal pyramids. The characteristics above showed that black-tailed gazelle kidneys possessed a strong reabsorption and hence promoting the production of high concentrated urine.

Assessment Of Differante Techniques For Oocyte Collection For Ivf In The Camel (Camelus dromedarius)

Majed A. AL-Fatlawy; Souhayla O .Hussain

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 76-88

The aims of the present study were to compare between the harvested methods of oocytes of she camel ovaries and their effect on the numbers of oocytes collection, Grad of oocytes and ratio of maturation oocytes from AL-Najaf slaughterhouse from December 2017 to January 2018 in rutting season . The oocytes numbers, graded of oocytes and the rate of maturation oocytes. The collection of oocytes was done by aspiration, slicing and slashing .One hundred ninety quality oocytes (190) were recovered from 90 ovaries from she camel at age (4-7) years. The study revealed that slicing method yielded significantly different (p<0.05) number of oocytes from the others methods as 77 oocytes distribution Grad A, B and C 25 (32.4%), 30 (38.9%), 22 (28.7%), respectively. While the aspiration method yielded 54 oocytes distribution Grad A, B and C 22(40.7%), 18(33.3%), 14(25.9%), respectively. The slashing method yielded 59 oocytes distributed grad A, B and C 23(38.9%), 20(33.9%), 16(27.11%).The study appearance the aspiration method was better than the slashing and slicing method which obtained higher quality oocytes maturation percentages and significant different( p<0.05) compared to the other methods. From the forty oocytes cultured in TCM- 199, 18 oocytes showed cumulus expansion with cytoplasmic density and first polar body, The percentages 45% (18/40 mature oocyte). The slashing method collection culture 43 oocytes 15 oocytes showed cumulus expansion and cytoplasm density and first polar body the percentage 34% (15/43). Slicing method collection in same medium, out of 55 oocytes cultured only 11 oocytes showed cumulus expansion with cytoplasm density and first polar body. The percentage was 20% (11/55). There was a significant difference (p<0.01) between different collected method.

Prevalence of Schistosomiasis among Patients attending hospitals in Babylon province

J.K. Ali; E.W. Kadhim

Kufa Journal For Veterinary Medical Sciences, Volume 9, Issue 1, Pages 89-92

Urine samples of 592 patients , attending hospitals were collected to detected prevalence of Schistosoma haematobium parasite in Babylon province . it's found that only 2 patients were infected (0.4%). The infected cases recorded in September / 2016 and June / 2017 ( Case in each month ) . The 2 positive cases were males out of 228 male patients (0.9%), while no infection found among females . one of the infected cases recorded among 84 individuals in the category 6-14 years (1.2%) , second case was among 44 individuals in the category 15-30 years (2.3%) . Symptoms of Schistosomiasis among some patients was studied